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Then a biotinylated detection antibody specific for Rat RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Human sCD14
Only those wells that contain Rat KIM-1
You can calculate the concentration of Human MBP-C/MBL2 in the samples by comparing the OD of the samples to the standard curve
The Human CK-18/KRT18 (Cytokeratin 18) ELISA kit uses the Sandwich-ELISA principle
Equine TGF-Beta3 ELISA Kit ALS9 Then a biotinylated detection antibodyThe Equine TGF Beta3 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Horse TGFb3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Horse TGFb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
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